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New Updated (on JUL/2017)
Brand-new Ortansia Multi-functional Imaging System (OMFIS) Version 2.0.100_SLM-beta is being developed. Version 1.1.100_AOI is now available in Dr. Shu Jia’s lab at Stony Brook University. The latest version is a LabVIEW based program which enables simultaneous imaging with different axial depths and is compatible with optical modulation with HOLOEYE VIS-16 SLM. For further information, please contact Xuanwen Hua. Finally, please note that the “Ortansia” mark is created and developed by Xuanwen Hua and please ask for permission for any usage and reference.
Updated (on OCT/2016)
My current work is to build and develop the technology of next-generation super-resolution microscopy. Applying the PSF engineering and deconvolution image processing to my researches, I’m trying to enhance the spatial resolution of conventional optical microscopy to break the diffraction limit of light. Such set-up might be able to realize sub-diffraction three-dimensional particle tracking.
Simultaneous Three-dimensional Tracking and Fluorescent Imaging of Microorganism Motility
Currently, although there are some researches on three-dimensional tracking and fluorescent imaging in the field of microorganism studies, the combination of both, that enable simultaneous tracking in three-dimensional scale while operating fluorescent imaging using CMOSs. By now, through our brief investigation we have known that the main difficulty of this project is to resolve the contradiction between the exposure time of imaging and the time of feedback and tracking. Hence, we are thinking that whether or not it would be possible for us to utilize defocused imaging method to collect image information in three dimensions so as to realize three-dimensional tracking and fluorescent imaging. During the entire processes, approaches to marking the microorganisms (e.g., E. coli) with fluorescent proteins should be involved to enable the observation of their appearances and movement states.
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Development of a new sub-diffraction-limit beam form with the design of 2D optical “magic carpet”
According to the article Toward the Optical “Magic Carpet”: Reducing the Divergence of a Light Sheet below the Diffraction Limit, we have known that with the application of two pairs of slits allow the light wave to propagate beyond Rayleigh range of a standard Gaussian beam. Here we are trying to move steps forward to design a “2D magic carpet”, so as to obtain a new form of light wave whose main lobe can propagate much farther than Gaussian beam under the diffraction limit in two dimensions with significantly suppressed side lobes. Such new wave form can provide better performances in tracking and imaging experiments with its long-propagating and sub-diffracting characteristics.
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